Detection of mycobacteria in coalho cheese sold in Northeastern Brazil

The aim of the present study was to detect and identify Mycobaterium spp. in 50 samples coalho cheese sold Northeast region Brazil. Of analyzed samples, 35 were produced by artisanal process, using raw milk, 15 originated from industrialized establishments that pasteurize milk. Conventional real-time nested PCR for rv2807 gene M. tuberculosis complex performed directly samples. Samples grown simultaneously Stonebrink medium, conventional bacterial isolates with primers flank differentiation 4 (DR4), specific bovis, mb400F. Bacterial negative RD4 subjected hsp65 Mycobacterium spp., subsequent DNA sequencing. cultures complex, but two (4%) process (with milk) exhibited identity lehmanii (Sequence ID: KY933786.1, identities: 312/363 [86%]); rutilum (sequence LT629971.1, 331/371 [89%]), showing be indicative environmental contamination. Non-tuberculous mycobacteria are emergent ubiquitous microorganisms; therefore, they deserve greater attention production chain, both terms Good Agricultural Practices (GAP) food Manufacturing (GMP).